Kaka-Manna

Seminar:

DNA Hybridization on Surfaces



Speaker: Fouzia Bano, SISSA International School of Advanced Studies, Trieste, Italy.

Venue: Centre for Solid State Physics, University of the Punjab, Lahore.

Time and Date: 7/28/2008 (month/day/year)

Download announcement


Introduction:


The controlled arrangement of DNA molecules on surfaces represents one challenging contribution of nanotechnology to biology and medicine. In particular, one of the open issues in the field of DNA-based sensors is detecting the hybridization process with high precision in a real-life biological environment. Towards this end, we have studied the hybridization of single stranded (ss)-DNA anchored on a gold surface using the increase in height of the molecules upon hybridization with a label free target which is due to the much larger rigidity of ds- vs. ss-DNA. Nano-scale ss-DNA patches are assembled within oligo-ethylene-glycol terminated alkylthiol self-assembled monolayer on a gold substrate using nanografting (an atomic force microscopy-based nanolithography technique). Differential height measurements indicate that ss-DNA nano-patches do not show significant increase in height upon hybridization with complementary strands in high density regime. Moreover, the advantage of this system for biosensors and genomics applications will be discussed briefly in the end.

curious101

nanai technology is the way forward there are so many ppl woking to sequence the human genome for less than $1000 and most are using nanopores, using the single strand DNA hybirdisation to solid surfaces and degrading each base off and reading from the electric current though the pore to see which base it is.

its a race for many companies now to sequence the genome for $1000 and a suitable time frame so that a patient can go to the octors and get their genome sequence quickly so doctor can see which medications would be best suited for the patient, but pharmacogenetics is another topic in itself wityh its own pro's and con's

desert bird

nice information , thanks

Fauzia is a Doctoral student at SISSA in the same field. Nanotech is now coming gradually to commercialization from labs and research.Hope to see drastic changes with new era tech in biomedical, pharmacy and hardware reduction by single electron transistors etc. Few scientists of Pakistan have already did some work in this field and many are working towards it in US, Europe etc.

Nanomaterials in drug delivery and cancer